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    • 专利摘要:
    The present disclosure discloses a screening method of a low-temperature-resistant Lycopersicon esculenlum variety, including: using a seed germination energy indeX under low-temperature stress as a selection indicator to screen a low-temperature- resistant Lycopersicon esculenlum variety. The method can be used to quickly, accurately, scientifically, and efficiently evaluate the low temperature resistance of Lycopersicon esculenlum varieties, which provides feasible technical indicators and detection methods for screening a low-temperature-resistant Lycopersicon esculenlum variety.
    公开号:NL2029148A
    申请号:NL2029148
    申请日:2021-09-08
    公开日:2021-10-22
    发明作者:Ma Xiaomei；Liu Xinqing；Guo Jiajin；Hu Yongjun；Ding Junyang；Tian Subo
    申请人:Shandong Shouguang Vegetable Ind Group Co Ltd；Shandong Shouguang Vegetable Seed Ind Group Co Ltd；
    IPC主号:
    专利说明:

    [0001] [0001] The present disclosure relates to a screening method of a low-temperature- resistant Lycopersicon esculentum variety, and belongs to the technical field of vegetables.BACKGROUND
    [0002] [0002] The selection and use of a low-temperature-resistant variety is the most effective way to avoid low-temperature damage on Lycopersicon esculentum. How to quickly and accurately screen out a low-temperature-resistant variety is an urgent problem to be solved in current greenhouse tomato production.
    [0003] [0003] Chinese patent CN111886963A discloses an evaluation method of low- temperature-resistant solanaceous vegetable varieties and discloses a screening method of a low-temperature-resistant Lycopersicon esculentum variety. In this patent, classification is conducted according to radicle lengths of germinated seeds, and a seed vigor index is calculated, thereby determining the low-temperature resistance of a Lycopersicon esculentum variety. However, the determination of an average radicle length of germinated seeds is easily interfered by ungerminated seeds. Moreover, the classification based on radicle lengths of germinated seeds involves uneasy practical operation, a large workload, room for large measurement errors, and has low accuracy.SUMMARY
    [0004] [0004] The technical problem to be solved by the present disclosure is to provide a screening method of a low-temperature-resistant Lycopersicon esculentum variety in view of the above problems, such that the purpose of the present disclosure to quickly, accurately, and easily evaluate the low-temperature resistance of varieties and guide the screening of a low-temperature-resistant Lycopersicon esculentum variety can be achieved.
    [0005] [0005] To solve the above technical problem, the present disclosure adopts the following technical solutions.
    [0006] [0006] The present disclosure provides a screening method of a low-temperature- resistant Lycopersicon esculentum variety, including: using a seed germination energy index at a low temperature as a selection indicator to screen the low-temperature- resistant Lycopersicon esculentum variety.
    [0007] [0007] The low-temperature-resistant Lycopersicon esculentum variety may have a seed germination energy index of < 0.10 under low-temperature stress.
    [0008] [0008] The seed germination energy index may be calculated according to the following formula:
    [0009] [0009] seed germination energy index = Xxa/(5Xx) = (Xpaotx a txeartx3a:+x4a4)/(5Xx)
    [0010] [0010] where
    [0011] [0011] xo, x1, X2, X3, and x4 each represent the number of seeds at a corresponding grade;
    [0012] [0012] ao, a1, a2, a3, and a4 each represent a germinated seed distribution grade;
    [0013] [0013] Xx represents the total number of sampled seeds;
    [0014] [0014] the germinated seed distribution grade is determined as follows: conducting a suitable temperature germination experiment to determine the average number of days (d) for seeds to complete germination at a suitable temperature, and conducting a staged low-temperature stress germination experiment to determine the germinated seed distribution grade according to the following specific criteria:
    [0015] [0015] grade 0: completing germination on day d or earlier;
    [0016] [0016] grade 1: completing germination on day (d+1);
    [0017] [0017] grade 2: completing germination on day (d+2);
    [0018] [0018] grade 3: completing germination on day (d+3); and
    [0019] [0019] grade 4: completing germination and ungerminating on day (d+4).
    [0020] [0020] The suitable temperature germination experiment may be conducted at 24°C to 26°C; and the staged low-temperature stress germination experiment may be conducted at 17°C to 19°C in a first stage and at 15.5°C to 16.5°C in a second stage.
    [0021] [0021] The first stage of the staged low-temperature stress germination experiment may involve a time period of day O to day 6 after seeds are placed in an artificial climate chamber (ACC).
    [0022] [0022] Preferably, the first stage may last for 5 d.
    [0023] [0023] The suitable temperature germination experiment may be conducted according to the following steps:
    [0024] [0024] 1) selecting seeds to be evaluated, disinfecting the seeds with a sodium hypochlorite solution, rinsing the seeds with tap water, and placing the seeds in a petri dish;
    [0025] [0025] 2) adding distilled water to the petri dish, and placing the petri dish in an ACC at 25+1°C to allow germination in the dark; and
    [0026] [0026] 3) when more than 80% of the seeds germinate, calculating the average number of days (d) for germinated seeds to complete germination.
    [0027] [0027] Preferably, the suitable temperature germination experiment may be conducted according to the following steps:
    [0028] [0028] 1) selecting 20 to 30 seeds to be evaluated, disinfecting the seeds with a 10% sodium hypochlorite solution for 20 min, rinsing the seeds with tap water 3 times, and placing the seeds in a ®9cm petri dish covered with a layer of filter paper;
    [0029] [0029] 2) adding 7 mL of distilled water to the petri dish, and placing the petri dish in an ACC at 25+1°C to allow germination in the dark;
    [0030] [0030] 3) after the seeds are placed in the ACC, timing, observing the seeds and replenishing water regularly every day, and recording the number of germinated seeds;
    [0031] [0031] 4) when more than 80% of the seeds germinate, calculating the average number of days (d) for germinated seeds to complete germination.
    [0032] [0032] The staged low-temperature stress germination experiment may be conducted according to the following steps:
    [0033] [0033] 1) selecting seeds to be evaluated, disinfecting the seeds with a sodium hypochlorite solution, rinsing the seeds with tap water, and placing the seeds in a petri dish; and
    [0034] [0034] 2) adding distilled water to the petri dish, and placing the petri dish in an ACC at 18£1°C to allow germination in the dark; and 5 d later, transferring the petri dish to an ACC at 164#0.5°C to allow further germination in the dark.
    [0035] [0035] Preferably, the staged low-temperature stress germination experiment may be conducted according to the following steps:
    [0036] [0036] 1) selecting 30 to 40 seeds to be evaluated, disinfecting the seeds with a 10% sodium hypochlorite solution for 20 min, rinsing the seeds with tap water 3 times, and placing the seeds in a @9cm petri dish covered with a layer of filter paper;
    [0037] [0037] 2) adding 7 mL of distilled water to the petri dish, and placing the petri dish in an ACC at 18+1°C to allow germination in the dark; and 5 d later, transferring the petri dish to an ACC at 16+0.5°C to allow further germination in the dark; and
    [0038] [0038] 3) after the seeds are placed in the ACC, timing, observing the seeds and replenishing distilled water regularly every day, and recording the number of germinated seeds.
    [0039] [0039] A radicle of the germinated seed may break through a seed coat by no less than 2 mm,
    [0040] [0040] Compared with the prior art, the present disclosure adopting the above technical solutions has the following advantages:
    [0041] [0041] The present disclosure provides feasible technical indicators and detection methods for the evaluation and screening of a low-temperature-resistant Lycopersicon esculentum variety. The seed germination energy index can be obtained through experimental visual observation and calculation, without manual measurement by a tool, which results in small workload, high efficiency, high accuracy, and easy operation.
    [0042] [0042] The present disclosure will be further described below with reference to specific implementations.DETAILED DESCRIPTION OF THE EMBODIMENTS
    [0043] [0043] The following examples are intended to illustrate the present disclosure, rather than to limit the protection scope of the present disclosure.
    [0044] [0044] Example 1 A screening method of a low-temperature-resistant Lycopersicon esculentum variety
    [0045] [0045] The screening method of a low-temperature-resistant Lycopersicon esculentum variety specifically included the following steps:
    [0046] [0046] In 2017, 5 Lycopersicon esculentum varieties were randomly selected on a seed market in Shouguang City and numbered 1, 2, 3, 4, and 5, and 60 seeds were randomly selected from each variety.
    [0047] [0047] Suitable temperature germination experiment: 20 seeds to be evaluated were randomly selected, disinfected with a 10% sodium hypochlorite solution for 20 min, rinsed with tap water 3 times, and then placed in a ®9cm petri dish covered with a layer of filter paper; 7 mL of distilled water was added to the petri dish, and then the petri dish was placed into an ACC to allow germination at 25+1°C in the dark, where after the
    [0048] [0048] Staged low-temperature stress germination experiment. 40 [Lycopersicon esculentum seeds to be evaluated were randomly selected, disinfected with a 10% sodium hypochlorite solution for 20 min, rinsed with tap water 3 times, and then placed in a petri dish (®9cm) covered with a layer of filter paper; 7 mL of distilled water was added to the petri dish, then the petri dish was placed in an ACC to allow germination at 18+1°C in the dark, and 5 d later, the temperature was adjusted from 18+1°C to 16+0.5°C to allow further germination in the dark, where the seeds were observed and distilled water was replenished regularly every day. After the seeds were placed in the ACC, the seeds were visually observed and the germination of seeds was recorded every day. A seed with a radicle breaking through a seed coat by 2 mm was determined as a germinated seed.
    [0049] [0049] The seed germination energy index was calculated according to the following formula:
    [0050] [0050] seed germination energy index = Xxa/(5Xx) = (Xoan+x1ar+x2a1+x3a3+X444)/(52x)
    [0051] [0051] where
    [0052] [0052] xo, x1, X2, x3, and x4 each represent the number of seeds at a corresponding grade;
    [0053] [0053] ao, ai, az, a3, and a4 each represent a germinated seed distribution grade; and
    [0054] [0054] >x represents the total number of sampled seeds.
    [0055] [0055] Through the staged low-temperature stress germination experiment, the germination time for each batch of sampled seeds placed at a low temperature was used to determine a germinated seed distribution grade. The germination time was counted from day 1 after the low temperature stress started, in a unit of d. Specific classification criteria were as follows:
    [0056] [0056] grade 0: completing germination on day d or earlier; |0057] grade 1: completing germination on day (d+1);
    [0058] [0058] grade 2: completing germination on day (d+2);
    [0059] [0059] grade 3: completing germination on day (d+3); and
    [0060] [0060] grade 4: completing germination and ungerminating on day (d+4).
    [0061] [0061] A result retained 2 digits after a decimal point.
    [0062] [0062] Specific test results were shown in the table below: Suitable- Number of seeds at a corresponding Variety germination germination | Grade | Grade | Grade | Grade | Grade energy index oo wijn EEE [vo jon] Msp mo [i [vo fio wp EERE me
    [0063] [0063] Specific evaluation results:
    [0064] [0064] No. 1: seed germination energy index: 0.13, not resistant to low temperature.
    [0065] [0065] No. 2: seed germination energy index: 0.18, not resistant to low temperature.
    [0066] [0066] No. 3: seed germination energy index: 0.09, resistant to low temperature.
    [0067] [0067] No. 4: seed germination energy index: 0.31, not resistant to low temperature.
    [0068] [0068] No. 5: seed germination energy index: 0.45, not resistant to low temperature.
    [0069] [0069] Lycopersicon esculentum No. 1 and No. 3 each were subjected to overwintering cultivation in a solar greenhouse A with prominent heat preservation (the lowest temperature at night was not lower than 13°C) and a solar greenhouse B with poor heat preservation (the lowest temperature at night was not lower than 6°C and not higher than 10°C), where except for different cultivation temperatures, management measures were exactly the same. Results: Lycopersicon esculentum No. 1 in the solar greenhouse A underwent no chilling damage and grew well, but Lycopersicon esculentum No. 1 in the solar greenhouse B underwent chilling damage and grew poorly; and Lycopersicon esculentum No. 3 in both the solar greenhouse A and the solar greenhouse B underwent no chilling damage, grew consistently, and had comparable yields. It shows that the screening result of the screening method of a low-temperature-resistant Lycopersicon esculentum variety is consistent with the actual situation, and thus the method can be used for the evaluation and screening of a low-temperature-resistant Lycopersicon esculentum variety.
    [0070] [0070] Finally, it should be noted that the above are merely preferred examples of the present disclosure and are not intended to limit the present disclosure. Although the present disclosure is described in detail with reference to the foregoing examples, a person skilled in the art can still make modifications to the technical solutions described in the foregoing examples, or make equivalent replacement to some technical features. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and scope of the present disclosure should be included within the protection scope of the present disclosure.
    权利要求:
    Claims (10)
    [1]
    A screening method of a low temperature resistant Lycopersicon esculentum variety comprising: using a seed germination energy index at a low temperature as a selection indicator to screen the low temperature resistant Lycopersicon esculentum variety.
    [2]
    The screening method of claim 1, wherein the low temperature resistant Lvcopersicon esculentum variety has a seed germination energy index of < 0.10 at low temperature stress.
    [3]
    The screening method of claim 1, wherein the seed germination energy index is calculated according to the following formula: seed germination energy index = Zxa/(55x) = (xoartx1artx2ar+x3a3+x4a4)/(52x) wherein X0, X1, X2, X3 and x4 each representing the number of seeds in a corresponding rank; ao, a1, az, a3 and a4 each represent a germinated seed distribution rate; ¥x represents the total number of seeds sampled; the germinated seed distribution rate is determined as follows: performing an appropriate temperature germination experiment to determine the average number of days (d) for seeds to complete germination at an appropriate temperature, and performing a phased low temperature germination experiment to determine the germinated seed distribution rate according to the following specific criteria: grade 0: germination completion on day d or earlier; grade 1: germination completion on day (d+1); grade 2: germination completion on day (d+2); grade 3: germination completion on day (d+3); and grade 4: germination completion and germination on day (d+4).
    [4]
    The screening method of claim 3, wherein the appropriate temperature germination experiment is performed at 24°C - 26°C; and the staged low temperature germination experiment is performed at 17°C - 19°C in a first phase and at 15.5°C - 16.5°C in a second phase.
    -9-
    [5]
    The screening method according to claim 4, wherein the first phase of the phased low temperature germination experiment comprises a time period from day 0 to day 6 after seeds are placed in an artificial climate chamber (ACC).
    [6]
    The screening method according to claim 3, wherein the appropriate temperature germination experiment is performed according to the following steps: 1) selecting seeds to be evaluated, disinfecting the seeds with a sodium hypochlorite solution, rinsing the seeds with tap water and placing the seeds in a petri dish; 2) adding distilled water to the petri dish and placing the petri dish in an ACC at 24°C - 26°C to allow germination in the dark; and 3) when more than 80% of the seeds germinate, calculate the average number of days (d) for germinated seeds to complete germination.
    [7]
    A screening method according to claim 3, wherein the staged low temperature germination experiment is performed according to the following steps: 1) selecting seeds to be evaluated, disinfecting the seeds with a sodium hypochlorite solution, rinsing the seeds with tap water and placing the seeds in a petri dish; 2) adding distilled water to the petri dish and placing the petri dish in an ACC at 17°C - 19°C to allow germination in the dark; and, 5 days later, transferring the petri dish to an ACC at 15.5°C - 16.5°C to allow further germination in the dark.
    [8]
    The screening method according to claim 6, wherein the appropriate temperature germination experiment is performed according to the following steps: 1) selecting 20 to 30 seeds to be evaluated, disinfecting the seeds for 20 min with a 10% sodium hypochlorite solution, rinsing the seeds 3 times with tap water and placing the seeds in a ® 9 cm petri dish that covers
    -10- is with a layer of filter paper; 2) adding 7 ml of distilled water to the petri dish and placing the petri dish in an ACC at 24°C - 26°C to allow germination in the dark; 3) After the seeds are placed in the ACC, time it, observe the seeds, and regularly replenish water every day, and record the number of germinated seeds; 4) When more than 80% of the seeds germinate, calculate the average number of days (d) for germinated seeds to complete germination.
    [9]
    The screening method according to claim 7, wherein the staged low temperature germination experiment is performed according to the following steps: 1) selecting from 30 to 40 seeds to evaluate, disinfecting the seeds with a 10% sodium hypochlorite solution for 20 minutes, rinsing the said 3 times seeds with tap water and placing the seeds in a © 9 cm petri dish covered with a layer of filter paper; 2) adding 7 ml of distilled water to the petri dish and placing the petri dish in an ACC at 17°C to 19°C to allow germination in the dark; and, 5 days later, transferring the petri dish to an ACC at 15.5°C - 16.5°C to allow further germination in the dark; and 3) after the seeds are placed in the ACC, timing, observing the seeds and regularly replenishing distilled water daily, and recording the number of seeds germinated.
    [10]
    The method of screening according to claims 1-9, wherein a radicle of the germinated seed breaks through a seed coat by at least 2 mm.
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    同族专利:
    公开号 | 公开日
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    CN111886963A|2020-07-30|2020-11-06|山东永盛农业发展有限公司|Method for evaluating low-temperature-resistant variety of solanaceous vegetables|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    CN202110148172|2021-02-03|
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